您是否(fou)希望懸(xuan)浮(fu)細(xi)胞(bao)的免(mian)疫熒光像(xiang)貼(tie)壁細(xi)胞(bao)一樣容(rong)易(yi)?現(xian)在(zai)是這樣!Shi-fix™(由Shikhar生(sheng)物技術公司制造)蓋滑允許懸(xuan)浮(fu)細(xi)胞(bao)分(fen)層或直(zhi)接作為(wei)單層生(sheng)長。簡單,無憂(you)的實(shi)驗方(fang)案,無需(xu)離(li)心(xin)柱即可將(jiang)懸(xuan)浮(fu)細(xi)胞(bao)固定到(dao)載玻片上。只(zhi)需(xu)將(jiang)細(xi)胞(bao)添加(jia)到(dao)蓋玻片中,等待30分(fen)鐘(zhong),用PBS洗滌未結合的細(xi)胞(bao)并繼續免(mian)疫染色(se)(或繼續培養(yang)以獲(huo)得所(suo)需(xu)的細(xi)胞(bao)密度(du))。
這些蓋(gai)玻片(pian)也可(ke)用于染色懸(xuan)浮(fu)細(xi)(xi)胞(bao)以進行(xing)顯(xian)微(wei)鏡(jing)檢(jian)查,或用于固定懸(xuan)浮(fu)細(xi)(xi)胞(bao)以進行(xing)共聚焦顯(xian)微(wei)鏡(jing)檢(jian)查。更(geng)重(zhong)要的是(shi),如果需要,您(nin)還(huan)可(ke)以在細(xi)(xi)胞(bao)附著(zhu)在蓋(gai)玻片(pian)上時刺激它們。您(nin)可(ke)以像貼壁細(xi)(xi)胞(bao)一樣處理(li)非(fei)貼壁細(xi)(xi)胞(bao)!
Do you wish immunofluorescence of suspension cells was as easy as with adherent cells? Now it is! Shi-fix™ (manufactured by Shikhar Biotech) cover-slips allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining (or continue the culture to obtain desired cell densities).
These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. You can work on non-adherent cells as if they were adherent!
SHI-FIX cover-slips玻片做的懸浮細胞免疫熒光實驗圖:
SHI-FIX cover-slips玻片的操作步驟:簡單!方便!!
SHI-FIX cover-slips玻片的產品訂購信息: